ABSTRACT
Background: staphylococcus aureus is an infrequent, but one of the most successful bacteria that associated with infertility and are able to spermatozoa immobilization and agglutination
Objective: the aim of present study was to determine the frequency of S. aureus in semen obtained from infertile male patients in northwest Iran
Materials and Methods: seminal fluids of 100 infertile men were evaluated. Standard semen examination was done according to World Health Organization guidelines. After isolation and identification of S. aureus isolates according to reference methods, determination of susceptibility against important antibiotics and polymerase chain reaction were performed to identify mecA and tst genes
Results: data obtained from the present study shows that 16% of infertile male patients were colonized by S. aureus. Ten [62.5%] of the individuals had abnormal seminal fluid sperm motility and morphology and three [18.8%] of them had an abnormal seminal fluid density, whereas after washing with albumin-saline declined to 5 [31.3%], 4 [25%] and 1 [6.3%], respectively. The antibiogram results showed that, except penicillin, other antibiotics have high activity on isolates. Regarding polymerase chain reaction results, mecA sequences were detected in 3 [18.7%] strains, whilst the tst gene encoding TSST-1 was not detected in any of clinical strains
Conclusion: it would appear that the S. aureus may be an additional negative factor worsening sperm quality and affecting male fertility. Therefore, it demands that all the patients attending in infertility treatment facilities be investigated thoroughly
ABSTRACT
In September 2007 national standard manual was finalized and officially announced as the minimal quality requirements for all medical laboratories in the country. Apart from auditing laboratories, Reference Health Laboratory has performed benchmarking auditing of medical laboratory network [surveys] in provinces. 12[th] benchmarks performed in Tehran and Alborz provinces, Iran in 2010 in three stages. We tried to compare different processes, their quality and accordance with national standard measures between public and private hospital laboratories. The assessment tool was a standardized checklist consists of 164 questions. Analyzing process show although in most cases implementing the standard requirements are more prominent in private laboratories, there is still a long way to complete fulfillment of requirements, and it takes a lot of effort. Differences between laboratories in public and private sectors especially in laboratory personnel and management process are significant. Probably lack of motivation, plays a key role in obtaining less desirable results in laboratories in public sectors